Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers.
نویسندگان
چکیده
Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.
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عنوان ژورنال:
- The Analyst
دوره 138 24 شماره
صفحات -
تاریخ انتشار 2013